acellular 脱细胞(596) 去细胞(218)   脱细胞     [Method]Acellular spinal cord(freeze thawing +3%sodiumdeoxycholate + DNaseI 、RNaseA)and fresh spinal cord of rats were implanted into paravertebral muscles of rats.     [方法]将脱细胞脊髓(冻融+3%脱氧胆酸钠+DNase,RNase消化)及新鲜大鼠脊髓分别植入SD大鼠椎旁肌内,于术后1～4周分别取材进行组织学观察,通过HE染色评价炎症反应程度; 短句来源     Objective To culture and proliferate bone marrow mesenchymal stem cells (BMSCs) from bone marrow of SD rat in vitro, and to construct tissue-engineered skin with the cultured cells as well as tissue-engineered acellular dermal matrix.     目的:体外培养扩增SD大鼠骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs),复合组织工程化脱细胞真皮基质构建组织工程皮肤,为进一步临床应用奠定基础。 短句来源     Results The cultured cells were observed growing and proliferating well in vitro. With the cells cultured in the acellular dermal matrix,tissue-engineered skin could be construted in virto.     结果:体外培养的SD大鼠骨髓间充质干细胞生长良好,传代扩增容易,组织工程化脱细胞真皮基质去细胞完全,骨髓间充质干细胞在脱细胞真皮基质中生长良好,可体外构建组织工程皮肤。 短句来源     Conclusion Constuction of tissue-engineered skin with BMSCs as well as tissue-engineered acellular dermal matrix in vitro is feasible.     结论:利用体外扩增培养的骨髓间充质干细胞及制备的组织工程化脱细胞真皮基质可以体外联合构建组织工程皮肤。 短句来源     Human Fibroblast Cell Seeding on Acellular Porcine Aorta Valves     静态与动态下脱细胞猪主动脉瓣种植人成纤维细胞初步研究 短句来源 更多          去细胞     Results The cultured cells were observed growing and proliferating well in vitro. With the cells cultured in the acellular dermal matrix,tissue-engineered skin could be construted in virto.     结果:体外培养的SD大鼠骨髓间充质干细胞生长良好,传代扩增容易,组织工程化脱细胞真皮基质去细胞完全,骨髓间充质干细胞在脱细胞真皮基质中生长良好,可体外构建组织工程皮肤。 短句来源     Repair of peripheral nerve defect by the acellular allogeneic nerve grafts in different length     去细胞异体神经材料修复不同长度周围神经缺损 短句来源     Experimental study on human acellular amniotic membrane as the substrate for cultivating human umbilical vein endotholical cells     “去细胞”羊膜基质种植人脐静脉内皮细胞的实验研究 短句来源     Repair of the Radial Defect of Rabbit by Acellular Bovine Cancellous Bone Combined with Bone Marrow Stem Cells     去细胞牛松质骨复合骨髓基质干细胞修复兔桡骨缺损 短句来源     Degradation of Acellular Porcine Aorta Valve     去细胞猪主动脉瓣膜基质支架材料降解观察 短句来源 更多          “acellular”译为未确定词的双语例句     The Role of 1, 25-dihydroxyvitamin D_3 as a Cellular Growth Factor in Bone Tissue Engineering     1，25-（OH）_2D_3作为生物活性因子在组织工程骨中的应用 短句来源     Construction the Tissue Engineering Bladder by Autologous Muscle Derived Satellite Cells and Xenogeneic Bladder Acellular Matrix     采用自体肌卫星细胞及异种膀胱无细胞基质构建组织工程膀胱的实验研究 短句来源     ③acellular matrix materials.     ③去细胞基质材料。 短句来源     ③Osteoblast+VECs group, which compounded the osteoblast+ VECs with acellular bone material.     ③成骨细胞+血管内皮细胞组:成骨细胞+血管内皮细胞与脱细胞基质骨材料复合。 短句来源     RESULTS: ①Isolation, amplification and identification of epidermal stem cells: Before the amplified cells seeded on the acellular epidermis, keratin 19 (K19) was identified with immunohistochemistry, and α6 and CD71 with flow cytometry.     结果:①表皮干细胞的分离、扩增及鉴定:在扩增的细胞拟接种于无细胞真皮前,进行角蛋白19免疫组织化学鉴定、以流式细胞术进行α6、CD71鉴定。 短句来源 更多        查询“acellular”译词为用户自定义的双语例句     我想查看译文中含有：的双语例句 为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法，我们为您准备了出自英文原文的大量英语例句，供您参考。   acellular Mollicutes are unique microorganisms characterized by a great extent for the reduction in genetic material, which retained the capability of independent division on acellular nutrient media.        Sclerotia of the acellular (true) slime mould Fuligo septica as a model to study melanization and anabiosis        Acellular (true) slime moulds (Myxomycetes) are capable of a transition to the stage of sclerotium - a dormant form of plasmodium produced under unfavourable environmental conditions.        Tissue-engineered graft constructed by self-derived cells and heterogeneous acellular matrix        The maximal load of acellular matrix was decreased and 20% lower than that of untreated thoracic aorta, but the maximal tensions between them were not different statistically and they had similar load-tension curves.        更多           This paper reports that pertussiscs phase Ⅰ was cultivated in a 500- liter fermentor using a semisynthetic medium containing 0.05% heptakis (dimethylbcyclodextrin).The protective antigens were purified from the culture supernatant and mixed with diphtheria and tetanus toxoied and absorbed with alumiunum compound to form DPTa.This preparation was stored at 4-8℃ for one year and two years.The potency of three antigens and toxicity of acellular pertussis vaccine were tested.Sinultaneously the preparation... This paper reports that pertussiscs phase Ⅰ was cultivated in a 500- liter fermentor using a semisynthetic medium containing 0.05% heptakis (dimethylbcyclodextrin).The protective antigens were purified from the culture supernatant and mixed with diphtheria and tetanus toxoied and absorbed with alumiunum compound to form DPTa.This preparation was stored at 4-8℃ for one year and two years.The potency of three antigens and toxicity of acellular pertussis vaccine were tested.Sinultaneously the preparation was incubated at 37℃ both three weeks and three months.Toxicity reversion of acellular pertussis vaccine was tested too.The tests showed that potency and toxicity of DPTa stored in one year and two years at 4-8℃ were stable,and toxicity revergion of acellular pertussis vaccine incubated at 37℃ both three weeks and three months was not found. 采用半综合液体培养基（含０．０５％ＭｅβＣＤ），大罐培养百日咳Ⅰ相ＣＳ菌制备的吸附无细胞百日咳菌苗、白喉、破伤风类毒素混合制剂（ＤＰＴａ）置４－８℃分别保存一年、保存两年，测三种抗原成分的效力及毒性，以及将该制剂于３７℃分别放置三周、三个月，测定有无毒性逆转。结果表明，该制剂质量稳定，无毒性逆转 In this reports,the effect was detected in preparaing reference under the different formalin solution,temperature and inactivating time.The results indicated that the agglutination titer of prepared reference can reach the orginal level of pertussis I phase serum,especially,the preparation under 0.1% formalin at 25 ℃ for 96-120 hours.The freedom from heat labile toxicity test is negative.BWDU/ml is 75.9-128.4,LPU/ml is 2.1-6.0,HSU/ml is 3.9-5.7,stability is good.This vaccine can be used as reference in acellular... In this reports,the effect was detected in preparaing reference under the different formalin solution,temperature and inactivating time.The results indicated that the agglutination titer of prepared reference can reach the orginal level of pertussis I phase serum,especially,the preparation under 0.1% formalin at 25 ℃ for 96-120 hours.The freedom from heat labile toxicity test is negative.BWDU/ml is 75.9-128.4,LPU/ml is 2.1-6.0,HSU/ml is 3.9-5.7,stability is good.This vaccine can be used as reference in acellular pertussis vaccine toxicity test after standardization. 将不同灭活条件下制备的无细胞百日咳毒性试验参考苗进行了检测。结果表明，以浓度０．１％Ｆｏｒｍａｌｉｎ溶液２５℃灭活９６－１２０小时制备的无细胞百日咳菌苗毒性试验参考苗，凝集效价仍可达到百日咳Ⅰ相血清原效价；不耐热毒素试验呈阴性；毒性试验ＢＷＤＵ／ｍｌ为７５．９－１２８．４；ＬＰＵ／ｍｌ为２．１－６．０；ＨＳＵ／ｍｌ为３．９－５．７；稳定性良好。该苗可标化作为无细胞百日咳菌苗毒性试验的参考标准 Objective:To investigate a method to remove cellular components from bovine pericardial tissue,resulting a scaffold for tissue engineering of heart valve or cardiovascular patch. Methods:A detergent and enzyme extraction was practiced in this study.HE staining was performed to confirm the removal of cells and Von Gieson staining,to show the integrity of collagen and elastin.The changes in tissue shrinkage temperature,and mechanical properties were also studied. Results:The cells were removed effectively... Objective:To investigate a method to remove cellular components from bovine pericardial tissue,resulting a scaffold for tissue engineering of heart valve or cardiovascular patch. Methods:A detergent and enzyme extraction was practiced in this study.HE staining was performed to confirm the removal of cells and Von Gieson staining,to show the integrity of collagen and elastin.The changes in tissue shrinkage temperature,and mechanical properties were also studied. Results:The cells were removed effectively from bovine pericardial tissue,while the collagen and elastin were kept intact.The mechanical properties remained unaltered.Only the tissue shrinkage temperature dropped insignificantly. Conclusion:The research work demonstrated an effective procedure to remove cells from bovine pericardial tissue while kept its mechanical properties.The acellular matrix needs to be further evaluated biochemically and ultrastructurally.This approach may eventually lead to the engineering of tissue heart valves repopulated with patients' own cells. 目的 :对牛心包材料进行了去污剂—酶联合脱细胞研究 ,为组织工程学方法研制生物瓣提供适合的生物材料。　　方法 :用去污剂—酶四步脱细胞方法脱除牛心包组织的细胞。　　结果 :该方法脱细胞效果良好 ,且能较好地保持胶原纤维和弹性纤维原有的排列和分布。脱细胞后牛心包材料的厚度、抗拉负荷、伸长率和热皱缩温度只有轻微的减少 ,而抗拉强度没有变化。　　结论 :去污剂—酶联合脱细胞法效果良好 ,可用于组织工程的进一步研究。   << 更多相关文摘      相关查询 acellular capillaries acellular cementum acellular matrix acellular trachea acellular bone acellular gland selective acellular acellular valve acellular dermis acellular pertussis acellular capillary acellular plant

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